By Marie A. Di Berardino (auth.), Paul J. Verma, Alan O. Trounson (eds.)
Nuclear move Protocols: mobile Reprogramming and Transgenesis is a complete overview of nuclear move know-how in vertebrates, geared toward reprogramming differentiated nuclei and effecting distinctive gene move. The authors offer with no trouble reproducible suggestions for the new release of cloned embryos and animals in a few key examine and commercially very important vertebrates. extra chapters supply substitute state-of-the-art equipment for nuclear move, reminiscent of zona-free nuclear move. Of mammoth profit are descriptions of tactics linked to cloning, resembling in vitro maturation of oocytes, activation and tradition of cloned embryos, upkeep of being pregnant, and neonatal care of clones.
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Additional resources for Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis
Transgenic animals. [DNLM: 1. Cell Nucleus--transplantation. 2. Cloning, Organism--methods. 3. Cell Differentiation--genetics. 4. Cell Nucleus--physiology. 5. Gene Transfer Techniques. QU 450 N964 2006] I. Verma, Paul J. II. Trounson, Alan. III. ) ; v. 348.
Of immense benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones. Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis provides an understanding of the factors involved in nuclear reprogramming, which is imperative for the success of cloning. A section dealing with such cloning-related issues as aging and normality of clones is also included, making this an essential comprehensive handbook for research and commercial laboratories involved in, or intending to work on, nuclear transfer.
Wash the ovaries with transportation medium (see Note 4). In Vitro Production of Bovine Embryos 39 3. Aspirate follicular fluid from 3- to 6-mm follicles using an 18-gage needle (Becton-Dickinson) on a 10-mL syringe (Becton-Dickinson; see Note 5). 4. Stand tubes containing follicular fluid at room temperature for 10–15 min and aspirate precipitate (see Note 6). Transfer the precipitate into a Petri dish (Fisher, 100 × 15 mm). 5. Centrifuge supernatant at 3000g during 10 min at room temperature to obtain clear follicular fluid.
Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis by Marie A. Di Berardino (auth.), Paul J. Verma, Alan O. Trounson (eds.)