By Curtis C. Harris, Benjamin F. Trump and Gary D. Stoner (Eds.)
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Extra info for Normal Human Tissue and Cell Culture B. Endocrine, Urogenital, and Gastrointestinal Systems
1. HUMAN ENDOMETRIUM IN ORGAN CULTURE 11 deterioration of both epithelial and stromal components was found. 0% albumin (Fig. 7) appeared viable although there was stromal deterioration and epithelial simplification. Female steroid sex hormone supplements have also been thought to benefit the maintenance of endometrial organ cultures. Whereas estradiol improved the appearance of endometrial organ cultures during short intervals of maintenance, cultures continuously exposed to medium containing estradiol had decreased viability with necrosis of stroma and low attenuated epithelium (Fig.
2. Primary normal human cervical epithelium in culture for 7 days. Bar represents 80 pm. 44 GEORGE D. WILBANKS et al. 3. Norman human cervical cells two weeks after second subcultivation. Note possible epithelioid replacement cells (see text). Bar represents 80 pm. , 1967). The results of these attempts are presented in Table IV. The charcoal was effective in removing most of the progesterone, all of the dehydrotestosterone, all of the testosterone, and a considerable amount of the hydroxyprogesterone.
Accurate assessment or comparison of data from these studies is difficult because the number of possible experimental variables to explain divergent results from apparently similar studies is too great. , 1976; Keay, 1978), and the formation of toxic photoproducts from the breakdown of riboflavin and tryptophan in tissue culture media by ordinary fluorescent room light (Wang, 1976), further complicate interpretation. For example, some studies reported the growth of epithelial cells from normal areas of abnormal cervices having known neoplastic lesions, while others reported results with normal cervices from patients who were pregnant or who had other benign lesions of the uterus.
Normal Human Tissue and Cell Culture B. Endocrine, Urogenital, and Gastrointestinal Systems by Curtis C. Harris, Benjamin F. Trump and Gary D. Stoner (Eds.)