Read e-book online Introductory Experiments on Biomolecules and their PDF

By Robert K. Delong, Qiongqiong Zhou

ISBN-10: 0128009691

ISBN-13: 9780128009697

Introductory Experiments on Biomolecules and their Interactions presents a unique method of educating biomolecules within the lab. whereas that includes the considered necessary basics, it additionally captures the author’s adventure in undefined, therefore offering distinct, up to date experiments which take the educational adventure one-step extra.

The textual content parallels lectures utilizing a regular biochemistry undergraduate textual content. in contrast to most modern lab manuals out there which easily emphasize an advent of suggestions, this lab guide presents scholars with possibilities to illustrate and end up the data and theories they examine from class.

  • Features quantitative research of RNA degradation by means of RNase
  • Contains challenge units, calculations, and references for every lab totally immersing scholars within the studying process
  • Includes guideline on tips on how to retain a lab computer and write a proper lab report
  • Provides hands-on engagement with the 4 significant kinds of biomolecules and “real-life and higher utilized” examples of molecular interactions

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Extra resources for Introductory Experiments on Biomolecules and their Interactions

Example text

Once these proteins are folded, it is the accessibility of the various amino acids in the protein and the functional groups present on the surface that determines points of contact and interactions with other biomolecules. Some examples include the formation of a hydrophobic domain that can be inserted into the membrane, an active site for interaction with its specific substrate in the case of enzymes, a leucine zipper or tract of arginine and lysine for interaction with DNA or RNA, and many others.

2006, Pearson Prentice Hall, 2012, Chapter 1. [2] Jeffrey Paradis, Kristen Spotz, Hands On Chemistry, McGraw-Hill, 2006. [3]  John F. Robyt, Bernard J. White, Biochemical Techniques: Theory and Practice, Brooks-Cole Publishing Company, 1987. [4] David S. Page, Principles of Biological Chemistry, Willard Grant Press, 1981, 1976. Notes EXPERIMENT 1 11 E X P E R I M E N T 2 Preparing Buffers at a Specific Molarity and pH SAFETY AND HAZARDS Small quantities of concentrated acids or bases (acetic acid, HCl, NaOH) are required to adjust the final pH of each buffer.

The H+ binding affinity is determined by the equilibrium constant (Kb or Ka), which is specific to each particular buffer reagent. You can essentially think of buffers as molecular sponges that are able to sponge up H+ at various concentrations. Besides maintaining pH value, buffers have other applications, as is the case with phosphate buffered saline (PBS). In addition to dissolving proteins and other biomolecules, the osmolality and ion concentrations of PBS usually match those of the human body, so it is isotonic and nontoxic to cells.

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Introductory Experiments on Biomolecules and their Interactions by Robert K. Delong, Qiongqiong Zhou


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