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2003) In vitro folding of alpha-helical membrane proteins. Biochimica et Biophysica Acta, 1610:57–62. 6. M. A. (2004) X-ray structure of a protein conducting channel. Nature, 427: 36–44. 7. , and Iwata, S. (2002) Purification and crystallization of the respiratory complex formate dehydrogenase-N from Escherichia coli. Acta Crystallographica, D58: 160–162. 8. , and Iwata, S. (2004) Architecture of the photosynthetic oxygen-evolving center. Science, 303: 1831–1838. 9. , and Iwata, S. (2003) Structure and mechanism of the lactose permease of Escherichia coli.

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2002) Acta Crystallographica, D58: 459–471. 45. H. W. (2004) Acta Crystallographica, B60: 412–421. 46. F. M. (1996) Journal of Applied Crystallography, 29: 584–587. 47. McFerrin, M. and Snell, E. (2002) Journal of Applied Crystallography, 35: 538–545. W. LESLIE AND HAROLD R. POWELL MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK Abstract: Processing diffraction data falls naturally into three distinct steps: First, determining an initial estimate of the unit cell and orientation of the crystal; second, obtaining refined values for these parameters; and third, integrating the diffraction images.

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