Baculovirus Expression Protocols - download pdf or read online

By Christopher D. Richardson

ISBN-10: 0896032728

ISBN-13: 9780896032729

Baculovirus Expression Protocols deals either commercial and university-based researchers a entire comilation of the most recent baculovirus ideas besides step by step directions and time-saving options. The contributors-leading specialists within the field-present the varied expression plasmids presently in use, advisor the reader during the means of producing and choosing recombinant virus, and describe particular examples of recombinant protein construction and purification. The emphasis is on substitute and less complicated screening innovations for the choice of recombinant baculovirus. The publication additionally surveys a few of the insect mobilephone strains at present appropriate with the baculovirus approach. Highlights contain creation of recombinant virus utilizing linearized DNA and vectors that include a b-galactosidase indicator and an entire checklist of expression vectors at the moment on hand. exact descriptions for the scale-up of protein construction utilizing spinner flasks, bioreactors, and bug larvae also are integrated. Baculovirus Expression Protocols bargains either commercial and university-based researchers a very good choice of reproducible, step by step laboratory protocols. it's going to instantly turn into integral for somebody operating with baculoviruses and their software within the expression of recombinant proteins in insect cells.

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73,3235-3240. 36. , and Pu, Z. (1992). Stimulation of thymidine kinase activity in baculovirus infected cells is not due t,o a virus coded enzyme. Arch Virol. 127,3 15-3 19. 37. Weyer. U. , and Possee, R. D. (1990) Analysis of very late gene expression by Autographa californica nuclear polyhedrosis virus and the further development of multiple expression vectors. J, Gen. Virol. 71, 1525-1534. 38. Stewart, L. M. , Merrywheather, A. , Cayley, P. , and Possee, R. D. (1991) Construction of an improved baculovirus insecticide containing an insect-specific toxin gene.

And Richardson, C. (1990) Synthesis of the membrane fusion and hemagglutinin proteins of measles virus, using a novel baculovirus vector containing the P-galactosidase gene. J. Virol. 64,37-50. 31. Hill-Perkins, M. S. and Possee, R. D. (1990) A baculovirus expression vector derived from the basic protein promoter of Autographa califomica nuclear polyhedrosis virus. J. Virol. 71,971-976. 32. Richardson, C. , and Meighen, E (1992). Bacterial luciferase produced with rapid screening baculovnus vectors is a sensitive reporter for infection of insect cells and larvae which contain a P-galactosidase mdicator gene.

J. Viral. 67,2664-267 1. CHAPTER3 Insect Cell-Culture in Serum-Containing Techniques Medium Stefan A Weiss, William G. Whitford, Stephen E Gomen, and Glenn I? Godwin 1. Introduction Almost 50 years ago, Grace reported the successful establishment of continuous cell lines from insects that were without defined biological and biophysical parameters (I). Since that time, the minimal distinctive parameters for the establishment, maintenance, and manipulation of invertebrate cell cultures have been identified and optimized (2,3).

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Baculovirus Expression Protocols by Christopher D. Richardson


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