Analytical Molecular Biology - download pdf or read online

By Peter Bedson, Helen Parkes, V (Ginny) Saunders, Sue Upton

ISBN-10: 0854044728

ISBN-13: 9780854044726

Introduces the problems of validation and caliber to the bioanalytical neighborhood, in particular addressing DNA-based analyses. Designed to elevate understanding of the standards that may impression the validity of DNA research and the construction of extra demanding situations which are linked to the research of genuine samples. Spiral.

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Additional resources for Analytical Molecular Biology

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Identify performance characteristics applic able to the method and analytical task. Typically, a quantitative anal ysis may require validation of sensitivity, precision, accuracy, bias, spec ficity, recovery, range and robustness. 5. e. specif city > sensitivity > range > recovery > precision > bias > accuracy. The most logical order will vary, depending on the technique, analytical I equirements and sample. 6. Assess performance characteristics of methc bdology using a consistent supply of well characterised, reference, stand; rd or spiked material.

Any deviation from expected results will require corrective action and the co-analysed batch of samples will require re-analysis. As a guide, QC samples should make up approximately 5% of the samples analysed; this could be decreased to 1-2% for robust high-throughput types of analyses or increased to 50% when carrying out complex ad hoc forensic analyses. Routine quality control is usually assessed inside an organisation. 4). Control charts can be used routinely to record data obtained from control analyses.

6. I S 0 3534-1. 7. Davies, C. 1994. Concepts. In: The Immunoassay Handbook (ed. ), pp. 83116. Stockton Press, New York. 8. Smith, M. 1994. Method evaluation. In: The Immunoassay Handbook (ed. ), pp. 256-262. Stockton Press, New York. 9. Pappas, M. G. 1994. Biotechnology product development. In: The Biotech Business Handbook, pp, 134-139. Humana Press, Totowa, NJ. 10. Youden, W. J. and Steiner, E. H. 1975. Statistical Manual of the Association of Official Analytical Chemists. AOAC, Washington. 11.

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Analytical Molecular Biology by Peter Bedson, Helen Parkes, V (Ginny) Saunders, Sue Upton

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