By E.W. Caspari (Ed.)
This quantity in a sequence on genetics, emphasizes the range of genetic experiences. Articles hide the filamentous fungus neurospora, biogenesis of yeast ribosomes, evolutionary genetics of fish, drosophila transposable parts and the dropophila gene zeste.
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Extra info for Advances in Genetics, Vol. 19
By sunlamp irradiation (Lawrence and Christensen, 1974) 1. Also, its frequencies are relatively high, often barely ten times rarer than meiotic ones. , 1975). This method has therefore been tried only once (for group IV; Kafer 1958), but not used further, and only intergenic crossing over is discussed here. In selected intergenic crossovers, only one of the two strands involved in an exchange is recovered. , 1974; Gingold and Ashworth, 1974; Katz and Kao, 1974). However, in systems that select by visual criteria it is possible, with suitable markers in repulsion, to recover all four strands: namely, one crossover and one parental each in two “reciprocal” diploid segregants, as “twin spots” (Stern, 1936 ; Wood and Kiifer, 1967) ; (Fig.
9, but heterozygous for several additional markers) was grown on media containing pfp, and conidia were “needle-plated” from 50 apparent or real sectors. Among these, 34 fpA-resistant, near-diploid segregants were obtained. They all showed one of the two expected phenotypes: (i) 20 of them were phenotypically indistinguishable from the pink, poorly conidiating but well growing, primary crossovers obtained previously when suAadE in coupling with T l ( 1 ; V I I ) was used for selection. These segregants produced the familiar two- or three-step patterns, with light green 2n VII intermediates and dark green diploid sectors; (ii) the other 14 represented such light-green secondary segregants, namely 2n VII trisomics, with the occasional darker diploid sectors.
Since mitotic crossovers from translocation heterozygotes are monosomic for one translocated segment and trisomic for the other, it depends very much on the size and genetic content of these segments, which primary crossovers are detectable and/or grow enough to produce further stable segregants. Reciprocal crossing-over in either of the two heteromorphic chromosome arms is expected to produce a twin pair of unbalanced crossovers. Such twins are reciprocal for their imbalance, each one being trisomic for that translocation segment for which the other is monosomic.
Advances in Genetics, Vol. 19 by E.W. Caspari (Ed.)